Peptide Blends: Reconstitution & Dosing Guide

Everything you need to know about peptide blends — what they are, how to reconstitute them, and how to calculate your dose when multiple peptides share one vial.

Compiled by PeptideWiki Editorial Team
In This Guide

What Are Peptide Blends?

A peptide blend is a single vial containing two or more research peptides that have been lyophilized (freeze-dried) together by the manufacturer. Instead of buying each peptide in its own vial, you get a pre-combined product — one vial, one reconstitution, one injection.

Blends exist because certain peptides are commonly used together. For example, BPC-157 and TB-500 are frequently paired for injury recovery research, so manufacturers offer them pre-combined in what the community calls the “Wolverine” blend. Similarly, CJC-1295 and Ipamorelin are often sold as a single blend for growth hormone research.

You may see blends referred to as “combination peptides,” “multi-peptide vials,” or simply by their nickname (Wolverine, GLOW, KLOW). Regardless of the name, the concept is the same: multiple peptides in one vial.

Blends vs. Stacks: What's the Difference?

These terms are frequently confused — even by vendors. Understanding the distinction is important because it affects how you reconstitute, dose, and manage your peptides.

FeatureBlendStack
DefinitionMultiple peptides in one vialSeparate vials used in the same protocol
Vial count1 vial2+ vials
Injections per dose1 injection delivers all peptides1 injection per peptide
Dose flexibilityFixed ratio — cannot adjust individuallyFull control over each peptide's dose
ConvenienceHigh — one reconstitution, one drawLower — separate reconstitutions and draws
Side effect isolationDifficult — can't identify which peptide caused itEasy — can stop one peptide independently
CostOften lower per vialHigher but more flexible

Reading a Blend Vial Label

Before you reconstitute a blend, you need to know exactly how much of each peptide is in the vial. Blend labels typically follow one of three formats:

Itemized Format

Most common and clearest

BPC-157 5mg

TB-500 5mg

Total with Breakdown

Shows total and individual

10mg Blend

(BPC-157 5mg + TB-500 5mg)

Ratio-Based

Requires math to decode

BPC-157/TB-500

1:1 Blend, 10mg total

What to Look For

  • Individual peptide amounts — the mg of EACH peptide, not just the total. This is the number you use for dosing math.
  • Total combined mg — useful for understanding the vial, but never use this number for dosing calculations.
  • Purity percentage — look for >98% purity on each peptide in the blend.
  • Lot or batch number — for traceability and quality verification against COA documents.

How to Reconstitute a Blend

The physical reconstitution process for a blend is identical to a single peptide vial. You add bacteriostatic water, let it dissolve, and refrigerate. The only difference is in the math that comes after — calculating per-peptide concentrations.

1

Swab the vial top with an alcohol wipe

Clean the rubber stopper to prevent contamination. Let it air dry.

2

Draw your chosen volume of bacteriostatic water

Choose a water volume that gives you a convenient per-peptide concentration. See the dosing math section below for guidance.

3

Inject water slowly along the vial wall

Aim the stream down the inside glass wall — never spray directly onto the powder. This protects the peptide bonds from mechanical damage.

4

Let it dissolve — never shake

Set the vial upright and wait 5-10 minutes. If needed, gently roll between your palms. The solution should become completely clear.

5

Label the vial with per-peptide concentrations

Write each peptide's concentration (e.g., "BPC: 2,500mcg/mL | TB: 2,500mcg/mL"), the date, and discard date (28 days).

Dosing Math for Blends

This is where blends differ from single peptides — and where most confusion happens. The key principle: calculate each peptide's concentration separately, using its individual mg amount (not the total).

Step 1: Per-Peptide Concentration

Concentration = Individual Peptide mg ÷ Water Volume (mL)

Calculate this for each peptide in the blend separately. Remember: 1mg = 1,000mcg.

Step 2: Dose Volume

Dose Volume (mL) = Target Dose (mcg) ÷ Concentration (mcg/mL)

Pick the peptide you're targeting and calculate the volume needed for your desired dose. The other peptides come along proportionally in the same draw.

Step 3: Convert to Syringe Units

Syringe Units = Dose Volume (mL) × 100

On a U-100 insulin syringe, 1 unit = 0.01mL. So 0.1mL = 10 units.

Think of it this way: when you draw 10 units from a 1:1 blend, that 10 units contains both peptides. You don't need to draw twice or double the volume. One draw, both peptides, done.

Worked Examples

Here are three fully worked-through examples using popular blends. Follow along with the math so you can apply the same process to any blend.

Example 1: Wolverine Blend (BPC-157 5mg / TB-500 5mg)

Reconstitute: Add 2mL bacteriostatic water to the vial.

  • BPC-157 concentration: 5mg ÷ 2mL = 2,500mcg/mL
  • TB-500 concentration: 5mg ÷ 2mL = 2,500mcg/mL

Target dose: 250mcg BPC-157

  • Volume: 250 ÷ 2,500 = 0.1mL = 10 units
  • That same 10 units also delivers 250mcg TB-500

Result: 1 injection of 10 units = 250mcg BPC-157 + 250mcg TB-500

Example 2: CJC/Ipa Blend (CJC-1295 2.5mg / Ipamorelin 2.5mg)

Reconstitute: Add 2.5mL bacteriostatic water to the vial.

  • CJC-1295 concentration: 2,500mcg ÷ 2.5mL = 1,000mcg/mL
  • Ipamorelin concentration: 2,500mcg ÷ 2.5mL = 1,000mcg/mL

Target dose: 100mcg CJC-1295

  • Volume: 100 ÷ 1,000 = 0.1mL = 10 units
  • That same 10 units also delivers 100mcg Ipamorelin

Result: 1 injection of 10 units = 100mcg CJC-1295 + 100mcg Ipamorelin

Example 3: GLOW Blend (BPC-157 10mg / TB-500 10mg / GHK-Cu 10mg)

Reconstitute: Add 3mL bacteriostatic water to the vial.

  • Each peptide concentration: 10mg ÷ 3mL ≈ 3,333mcg/mL

Target dose: 500mcg BPC-157

  • Volume: 500 ÷ 3,333 = 0.15mL = 15 units
  • That same 15 units also delivers 500mcg TB-500 + 500mcg GHK-Cu

Result: 1 injection of 15 units = 500mcg BPC-157 + 500mcg TB-500 + 500mcg GHK-Cu

Skip the Math — Use Our Calculator

Enter your blend's individual peptide amounts, water volume, and desired dose — get instant syringe unit calculations.

The Fixed-Ratio Constraint

The most important limitation of peptide blends is that you cannot adjust individual peptide doses independently. Every draw delivers all peptides in the manufacturer's fixed ratio. This is fine when the ratio matches your protocol — and a problem when it doesn't.

When the Ratio Works

In a 1:1 Wolverine blend, if your protocol calls for equal doses of BPC-157 and TB-500 (for example, 250mcg of each), the blend is perfect. One draw gives you exactly what you need for both peptides.

When the Ratio Doesn't Work

If your protocol calls for 500mcg of BPC-157 but only 250mcg of TB-500, a 1:1 blend cannot deliver that. Drawing enough for 500mcg BPC automatically gives you 500mcg TB-500 as well. You have three options:

  • Accept the fixed ratio — take the extra TB-500 and adjust your expectations
  • Use individual vials instead — buy BPC-157 and TB-500 separately for full dose control
  • Find a blend with a different ratio — some vendors offer non-equal ratios

Non-Equal Ratios

Some blends intentionally use unequal ratios. The KLOW blend, for example, contains significantly more GHK-Cu (50mg) than the other peptides (10mg each) because GHK-Cu is typically used at higher doses. In these blends, the ratio is designed to approximate the different dose requirements of each peptide — but it still can't be adjusted after purchase.

Blends vs. Individual Peptides: Pros & Cons

Deciding between a blend and individual vials depends on your priorities — convenience vs. flexibility, simplicity vs. control.

Blend Advantages

  • One vial, one reconstitution, one injection
  • Fewer needle sticks per session
  • Simpler protocol — easier to follow consistently
  • Often lower cost than buying each peptide separately
  • Less refrigerator space needed
  • Ideal for beginners running standard protocols

Blend Disadvantages

  • Fixed ratio — cannot adjust individual peptide doses
  • Cannot isolate which peptide is causing side effects
  • Harder to verify purity of each peptide via third-party testing
  • Shelf life determined by the least stable peptide
  • Cannot run one peptide longer or shorter than the others
  • Less flexible for advanced or customized protocols

Quick Decision Guide

Choose a Blend When...

  • Your protocol uses equal doses of each peptide
  • You value convenience and fewer injections
  • You're running a well-established, standard protocol
  • You're a beginner keeping things simple
  • The blend ratio matches your target doses

Choose Individual Vials When...

  • You need different doses of each peptide
  • You want to titrate (adjust) one peptide independently
  • You need to isolate side effects to a specific peptide
  • You plan to run one peptide longer than the others
  • You require third-party purity testing per peptide

Storage & Shelf Life

Blend storage follows the same principles as individual peptides, with one important addition: the least stable peptide in the blend determines the shelf life of the entire vial.

Lyophilized (Before Reconstitution)

Unreconstituted blend vials are stable under the same conditions as individual peptides. Store in the freezer (−20°C) for maximum long-term stability, or in the refrigerator (2-8°C) for shorter-term storage. Keep away from light, heat, and moisture. A sealed lyophilized blend can remain stable for months (refrigerated) to years (frozen).

Reconstituted (After Adding Water)

Store reconstituted blends at 2-8°C (standard refrigerator temperature). With bacteriostatic water, use within 28 days. With sterile water, use within 24 hours. Never freeze a reconstituted blend — the freeze-thaw cycle damages peptide chains and is irreversible.

Blend-Specific Considerations

  • Weakest-link rule: If one peptide in the blend degrades after 14 days in solution while another is stable for 28 days, treat the entire blend as having a 14-day shelf life.
  • Clarity checks: Inspect the solution before each use. If it was clear after reconstitution and has become cloudy, one or more peptides may have aggregated — discard the entire vial.
  • Light protection: Some peptides (especially those containing tryptophan, tyrosine, or methionine residues) are photosensitive. If any peptide in the blend is light-sensitive, protect the entire vial from light exposure.

For a complete guide to peptide storage, temperature requirements, and signs of degradation, see our Peptide Storage & Shelf Life Guide.

Common Mistakes to Avoid

Blends introduce a few unique pitfalls beyond standard peptide handling. Most of these come down to one issue: not understanding that each peptide must be tracked individually.

Dosing based on total mg instead of per-peptide mg

A 10mg blend of 5mg BPC + 5mg TB-500 contains 5mg of each — not 10mg of each. Dosing based on 10mg total leads to double the intended concentration in your calculations.

Adding water based on total combined mg

The amount of water you add should be based on your target per-peptide concentration, not the sum of all peptides. Calculate water volume using the individual peptide you're targeting for dosing convenience.

Expecting to adjust individual peptide doses independently

In a blend, every peptide comes out in the same draw at the manufacturer's fixed ratio. If you need 500mcg of one and 250mcg of another, a 1:1 blend cannot deliver that — use individual vials instead.

Not calculating per-peptide concentration after reconstitution

Each peptide has its own concentration based on its individual mg amount. Skipping this step means you won't know how much of each peptide you're actually drawing per dose.

Assuming all blends with the same name have the same amounts

'Wolverine' is a nickname, not a standard. Different vendors may use different mg amounts or ratios. Always read the label before reconstituting.

Using the longest-stable peptide's shelf life for the whole blend

The peptide with the shortest reconstituted stability determines the shelf life of the entire vial. If one peptide degrades faster, the whole blend should be discarded on that timeline.

Shaking the vial to dissolve the blend

Shaking denatures peptide chains through mechanical stress. This is especially problematic with blends because multiple peptides means more molecules vulnerable to aggregation at foam boundaries.

Not verifying blend ratios match your dosing protocol before purchasing

If your protocol calls for different doses of each peptide, a blend with a fixed ratio may not work. Check the math before buying to confirm the blend ratio aligns with your intended doses.

Confusing 'blend' with 'stack' when reading protocols

A protocol written for a stack (separate vials) may specify different doses for each peptide. Applying those doses directly to a blend doesn't work because blends deliver peptides at a fixed ratio.

Drawing dose with the same needle used to add bacteriostatic water

The needle used for reconstitution can core the rubber stopper, introducing tiny rubber particles into the solution. Always use a fresh needle for drawing individual doses.

Frequently Asked Questions

Next Steps

Now that you understand how peptide blends work, explore these resources to continue your research.

Calculate Your Dose

Use our peptide dosage calculator to convert mg to mcg and find your syringe units for any blend.

Open Calculator

Reconstitution Guide

Full step-by-step reconstitution instructions with bacteriostatic water, syringe education, and troubleshooting.

Read Guide

Browse Peptide Directory

Research individual peptides — detailed profiles, mechanisms, and dosing protocols for 50+ compounds.

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Medical Disclaimer: This guide is for educational and informational purposes only. It is not medical advice and should not be used as a substitute for professional medical consultation, diagnosis, or treatment. Peptides discussed in this guide are referenced in the context of published research and are not approved for human therapeutic use unless otherwise noted. Always consult a qualified healthcare provider before beginning any new health protocol. PeptideWiki does not sell peptides or endorse any specific vendor or product.