ACTH (Cosyntropin)

Hormones & Endocrine (Non-GH)
Phase 4
FDA Approved

Cosyntropin is a synthetic version of the first 24 building blocks of the body's natural stress hormone ACTH. It is used as an FDA-approved diagnostic tool (Cortrosyn) to test whether the adrenal glands are working properly, and is also used to treat certain conditions like infantile spasms.

Last updated March 6, 2026
173 PubMed-Verified Studies
Moderate
Research-Driven Dosage Reports
RouteIntravenous (IV) · Intramuscular (IM)
Dose250 mcg (standard) or 1 mcg (low-dose test)
FrequencySingle dose (diagnostic) or per protocol (therapeutic)
DurationSingle use (diagnostic) or 2–12 weeks (therapeutic)

Based on published research literature.

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Overview of ACTH (Cosyntropin)

Binds MC2R receptors on adrenal cortex cells to stimulate cortisol and corticosteroid synthesis, used diagnostically to assess adrenal reserve and therapeutically for specific conditions.

Adrenal stimulation

Binds melanocortin 2 receptor (MC2R) on zona fasciculata cells of the adrenal cortex, activating adenylyl cyclase and cAMP-dependent protein kinase A signaling.

Stimulates acute cortisol synthesis by promoting cholesterol transport into mitochondria via StAR protein upregulation.

Triggers production of cortisol, corticosterone, and adrenal androgens in a dose-dependent manner.

Diagnostic use

Standard diagnostic tool: 250 mcg IV/IM with cortisol measured at 30 and 60 minutes. Subnormal cortisol response indicates adrenal insufficiency.

Low-dose (1 mcg) protocol may be more sensitive for detecting partial adrenal insufficiency (proposed).

Therapeutic applications

FDA-approved for diagnostic use; ACTH gel (repository corticotropin injection) is FDA-approved for infantile spasms, nephrotic syndrome, and other conditions.

Stimulates adrenal production of multiple steroids, providing a broader hormonal response than exogenous cortisol alone

Read Full ACTH (Cosyntropin) Dosage Guide

Research-backed dosing protocols, timing, and administration details

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Hormones & Endocrine (Non-GH)